Composite

Part:BBa_K4115023

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)


J23101-B0034-sfGFP(with LVA)-B0015

The superfolder GFP with an LVA degradation tag is expressed by a constitutive promoter J23101 in this part.

Usage and Biology

It was used as one of the controls in starvation promoter characterization experiments.
Usually, the higher the external carbon source or nitrogen source, the higher the expression level.
The introduction of a degradation tag can help to eliminate the accumulation of expression over time, reduce the excessively bright fluorescence, and also make the sensitivity of starvation promoters better reflected.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None