![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4115023
Designed by: Kaijun Wang Group: iGEM22_ShanghaiTech_China (2022-09-30)
J23101-B0034-sfGFP(with LVA)-B0015
The superfolder GFP with an LVA degradation tag is expressed by a constitutive promoter J23101 in this part.
Usage and Biology
It was used as one of the controls in starvation promoter characterization experiments.
Usually, the higher the external carbon source or nitrogen source, the higher the expression level.
The introduction of a degradation tag can help to eliminate the accumulation of expression over time, reduce the excessively bright fluorescence, and also make the sensitivity of starvation promoters better reflected.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |